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pgex 4t 3 vector  (Cytiva Europe)


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    Structured Review

    Cytiva Europe pgex 4t 3 vector
    Pgex 4t 3 Vector, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 94/100, based on 43 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pgex+4t+3+vector/pGEX+Vectors+PGEX-4T-3/pmc13151057-99-16-18
    Average 94 stars, based on 43 article reviews
    pgex 4t 3 vector - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: GRIM-19 Disrupts E6/E6AP Complex to Rescue p53 and Induce Apoptosis in Cervical Cancers
    Article Snippet: .. The PCR products were inserted into a BamHI/Xhol -digested pGEX-4T-3 vector (GE Healthcare, Piscataway, NJ, USA) to construct pGST-G19. ..

    Article Title: GRIM-19 Disrupts E6/E6AP Complex to Rescue p53 and Induce Apoptosis in Cervical Cancers
    Article Snippet: .. The PCR products were inserted into a BamHI/XhoI -digested pGEX-4T-3 vector (GE Healthcare), and the recombinant plasmid was named as pGST-CA-E6AP. ..

    Article Title: Identification of Sphingomyelinase on the Surface of Chlamydia pneumoniae : Possible Role in the Entry into Its Host Cells
    Article Snippet: Genomic DNA was prepared from C. pneumoniae using MagNA Pure Compact nucleic acid isolation kit (Roche Applied Science). .. The CPn0300 was amplified by PCR and cloned into the BamHI-XhoI site of pGEX-4T-3 vector (GE Healthcare Life Sciences, Uppsala Sweden). ..

    Plasmid Preparation:

    Article Title: GRIM-19 Disrupts E6/E6AP Complex to Rescue p53 and Induce Apoptosis in Cervical Cancers
    Article Snippet: .. The PCR products were inserted into a BamHI/Xhol -digested pGEX-4T-3 vector (GE Healthcare, Piscataway, NJ, USA) to construct pGST-G19. ..

    Article Title: GRIM-19 Disrupts E6/E6AP Complex to Rescue p53 and Induce Apoptosis in Cervical Cancers
    Article Snippet: .. The PCR products were inserted into a BamHI/XhoI -digested pGEX-4T-3 vector (GE Healthcare), and the recombinant plasmid was named as pGST-CA-E6AP. ..

    Article Title: Development of Antagonist Peptides of the Crk/CrkL SH2 Domains by Modifying the Peripheral Amino Acid Residues of the YXXP Motif
    Article Snippet: .. Mouse cDNA sequences of CrkII, CrkI, and CrkL with the FLAG tag were subcloned into the pGEX-4T-3 vector (GE Healthcare). .. BL21 (DE3) competent cells (New England Biolabs) were transformed with the plasmids, and expression of the GST-fusion proteins was induced by adding isopropyl β- d -1-thiogalactopyranoside to the bacterial culture.

    Article Title: Regulation of cargo exocytosis by a Reps1-Ralbp1-RalA module
    Article Snippet: REPS1 and RALBP1 were subcloned into the pGEX-4T-3 vector (GE Healthcare). .. REPS1 and RALBP1 were subcloned into the pGEX-4T-3 vector (GE Healthcare). .. Recombinant proteins were expressed in BL21 (DE3) Escherichia coli (Stratagene, #230132) as previously described ( , ).

    Article Title: Adaptor Proteins Intersectin 1 and 2 Bind Similar Proline-Rich Ligands but Are Differentially Recognized by SH2 Domain-Containing Proteins
    Article Snippet: The coding region of the cytoplasmic domain of Sema6A (residues 650–1021) was subcloned into the pcDNA4His/Max vector (Invitrogen) with an Omni tag. .. Sequences encoding the SH2 domains of Grb2 (residues 57–157), Crk (residues 5–127), Itk (residues 246–363), Fgr (residues 136–251), Fyn (residues 143–255), Abl1 (residues 140–244), PI3KR1-N (residues 327–437), PI3KR1-C (residues 617–724) and PLCg1 (residues 542–766) were subcloned into the pGEX-4T-3 vector (GE Healthcare). ..

    Article Title: Identification of Sphingomyelinase on the Surface of Chlamydia pneumoniae : Possible Role in the Entry into Its Host Cells
    Article Snippet: Genomic DNA was prepared from C. pneumoniae using MagNA Pure Compact nucleic acid isolation kit (Roche Applied Science). .. The CPn0300 was amplified by PCR and cloned into the BamHI-XhoI site of pGEX-4T-3 vector (GE Healthcare Life Sciences, Uppsala Sweden). ..

    Article Title: Regulation of AP1 adaptor assembly by the bi-handed chaperone MEA1.
    Article Snippet: .. To expressMEA1 in E. coli, HumanMEA1was subcloned into the BamHI and XhoI sites of the pGEX-4T-3 vector (GE Healthcare) and the pRSF-Duet-SUMO expression vector59,60. ..

    Construct:

    Article Title: GRIM-19 Disrupts E6/E6AP Complex to Rescue p53 and Induce Apoptosis in Cervical Cancers
    Article Snippet: .. The PCR products were inserted into a BamHI/Xhol -digested pGEX-4T-3 vector (GE Healthcare, Piscataway, NJ, USA) to construct pGST-G19. ..

    Recombinant:

    Article Title: GRIM-19 Disrupts E6/E6AP Complex to Rescue p53 and Induce Apoptosis in Cervical Cancers
    Article Snippet: .. The PCR products were inserted into a BamHI/XhoI -digested pGEX-4T-3 vector (GE Healthcare), and the recombinant plasmid was named as pGST-CA-E6AP. ..

    FLAG-tag:

    Article Title: Development of Antagonist Peptides of the Crk/CrkL SH2 Domains by Modifying the Peripheral Amino Acid Residues of the YXXP Motif
    Article Snippet: .. Mouse cDNA sequences of CrkII, CrkI, and CrkL with the FLAG tag were subcloned into the pGEX-4T-3 vector (GE Healthcare). .. BL21 (DE3) competent cells (New England Biolabs) were transformed with the plasmids, and expression of the GST-fusion proteins was induced by adding isopropyl β- d -1-thiogalactopyranoside to the bacterial culture.

    Amplification:

    Article Title: Identification of Sphingomyelinase on the Surface of Chlamydia pneumoniae : Possible Role in the Entry into Its Host Cells
    Article Snippet: Genomic DNA was prepared from C. pneumoniae using MagNA Pure Compact nucleic acid isolation kit (Roche Applied Science). .. The CPn0300 was amplified by PCR and cloned into the BamHI-XhoI site of pGEX-4T-3 vector (GE Healthcare Life Sciences, Uppsala Sweden). ..

    Clone Assay:

    Article Title: Identification of Sphingomyelinase on the Surface of Chlamydia pneumoniae : Possible Role in the Entry into Its Host Cells
    Article Snippet: Genomic DNA was prepared from C. pneumoniae using MagNA Pure Compact nucleic acid isolation kit (Roche Applied Science). .. The CPn0300 was amplified by PCR and cloned into the BamHI-XhoI site of pGEX-4T-3 vector (GE Healthcare Life Sciences, Uppsala Sweden). ..

    Expressing:

    Article Title: Regulation of AP1 adaptor assembly by the bi-handed chaperone MEA1.
    Article Snippet: .. To expressMEA1 in E. coli, HumanMEA1was subcloned into the BamHI and XhoI sites of the pGEX-4T-3 vector (GE Healthcare) and the pRSF-Duet-SUMO expression vector59,60. ..



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